Science2013-09-05 11:30 PM

Elongation Factor G Bound to the Ribosome in an Intermediate State of Translocation

A key step of translation by the ribosome is translocation, which involves the movement of messenger RNA (mRNA) and transfer RNA (tRNA) with respect to the ribosome. This allows a new round of protein chain elongation by placing the next mRNA codon in the A site of the 30S subunit. Translocation proceeds through an intermediate state in which the acceptor ends of the tRNAs have moved with respect to the 50S subunit but not the 30S subunit, to form hybrid states. The guanosine triphosphatase (GTPase) elongation factor G (EF-G) catalyzes the subsequent movement of mRNA and tRNA with respect to the 30S subunit. Here, we present a crystal structure at 3 angstrom resolution of the Thermus thermophilus ribosome with a tRNA in the hybrid P/E state bound to EF-G with a GTP analog. The structure provides insights into structural changes that facilitate translocation and suggests a common GTPase mechanism for EF-G and elongation factor Tu.

KEYWORDS

SHARE & LIKE

COMMENTS

ABOUT THE AUTHOR

Science

Science Magazine

0 Following 18 Fans 0 Projects 236 Articles

SIMILAR ARTICLES

AbstractThe conservation of sleep across all animal species suggests that sleep serves a vital function. We here report that sleep has a critical funct

Read More

Introduction Recent decades have seen a major international effort to inventory tree communities in the Amazon Basin and Guiana Shield (Amazonia), but

Read More

IntroductionInitiation of protein synthesis is a key step in the control of gene expression. In eukaryotes, initiation is a highly complex process that

Read More

Abstract For decades, social psychological theories have posited that the automatic processes captured by implicit measures have implications for soci

Read More

论文摘要动物界最复杂的眼睛可以在物种口足类甲壳动物(濑尿虾)中找到,其中一些有12种不同类型的光感受器,每种各取样较窄的一组波长,范围包含了深紫外光到远红光(300到720纳米)。在功能上,这种彩色复杂性被视作是谜。当3种或4种色彩通道对色彩辨认来说已经足够时,为何要使用12种色彩通道?对口足类动物行

Read More

论文摘要 首先,在一篇“评论”文章中,Thomas Piketty 和 Emmanuel Saez用“世界顶级收入数据库(WTID)”来比较这两个数值多年来是如何在欧洲和美国演变的;WTID包括了一个世纪之久的有关全球收入的数据以及2个世纪之久的有关个人财富的数据。据研究人员披露,在20世纪之交的时候

Read More

IntroductionCensorship has a long history in China, extending from the efforts of Emperor Qin to burn Confucian texts in the third century BCE to the c

Read More

Many animals, on air, water, or land, navigate in three-dimensional (3D) environments, yet it remains unclear how brain circuits encode the animal's 3D

Read More

Many animals, on air, water, or land, navigate in three-dimensional (3D) environments, yet it remains unclear how brain circuits encode the animal's 3D

Read More

Like mammalian neurons, Caenorhabditis elegans neurons lose axon regeneration ability as they age, but it is not known why. Here, we report that let-7

Read More