生物技术-自然2013-09-05 2:58 AM

Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay : Nature Biotechnology : Nature Publishing Group

It has been hypothesized that carcinoma metastasis is initiated by a subpopulation of circulating tumor cells (CTCs) found in the blood of patients. However, although the presence of CTCs is an indicator of poor prognosis in several carcinoma entities, the existence and phenotype of metastasis-initiating cells (MICs) among CTCs has not been experimentally demonstrated. Here we developed a xenograft assay and used it to show that primary human luminal breast cancer CTCs contain MICs that give rise to bone, lung and liver metastases in mice. These MIC-containing CTC populations expressed EPCAM, CD44, CD47 and MET. In a small cohort of patients with metastases, the number of EPCAM+CD44+CD47+MET+ CTCs, but not of bulk EPCAM+ CTCs, correlated with lower overall survival and increased number of metastasic sites. These data describe functional circulating MICs and associated markers, which may aid the design of better tools to diagnose and treat metastatic breast cancer.

KEYWORDS

SHARE & LIKE

COMMENTS

ABOUT THE AUTHOR

生物技术-自然

0 Following 0 Fans 0 Projects 12 Articles

SIMILAR ARTICLES

Reference genomes are required to understand the diverse roles of microorganisms in ecology, evolution, human and animal health, but most species remai

Read More

A low-error 16S ribosomal RNA amplicon sequencing method, in combination with whole-genome sequencing of >500 cultured isolates, was used to characteri

Read More

Delivery of short interfering RNAs (siRNAs) remains a key challenge in the development of RNA interference (RNAi) therapeutics. A better understanding

Read More

New high-dimensional, single-cell technologies offer unprecedented resolution in the analysis of heterogeneous tissues. However, because these technolo

Read More

In the version of this article initially published, there were two errors in the discussion of epigenetic marks on page 854. In sentence 4, paragraph 2

Read More

Genetic mutations causing human disease are conventionally thought to be inherited through the germ line from one’s parents and present in all somatic

Read More

Cell-based therapies for myelin disorders, such as multiple sclerosis and leukodystrophies, require technologies to generate functional oligodendrocyte

Read More

Transplantation of oligodendrocyte precursor cells (OPCs) is a promising potential therapeutic strategy for diseases affecting myelin. However, the der

Read More

It is currently not possible to predict which epitopes will be recognized by T cells in different individuals. This is a barrier to the thorough analys

Read More

Dysfunction of basal forebrain cholinergic neurons (BFCNs) and γ-aminobutyric acid (GABA) interneurons, derived from medial ganglionic eminence (MGE),

Read More