柳叶刀2013-12-27 8:55 PM

Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study : The Lancet Infectious Diseases 中东呼吸综合症冠状病毒中和血清抗体在单峰骆驼体内发现:比较血清学研究

Abstract Background A new betacoronavirus—Middle East respiratory syndrome coronavirus (MERS-CoV)—has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. 背景 在严重急性呼吸道感染患者中已发现了一种新型β- 冠状病毒,即中东呼吸综合征冠状病毒(Middle East respiratory syndrome coronavirus,MERS-CoV)。虽然相关病毒可感染蝙蝠,但分子时钟分析尚无法确定MERS-CoV 的直接来源。暴露史调查显示患者曾接触过单峰骆驼或山羊。因此,本研究通过评价牲畜群的血清特异性抗体来研究MERS-CoV 可能的动物宿主。 Methods We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. 方法 血清样本采自中东(阿曼)和其他地区(西班牙、荷兰和智利)的动物,包括牛(80 头)、绵羊(40只)、山羊(40 只)、单峰骆驼(155 头)以及其他类型的骆驼(34 头),我们利用蛋白质微阵列通过使用 MERS - CoV棘突蛋白的受体结合S1亚基,严重急性呼吸综合征冠状病毒和人类冠状病毒OC43测试了这些血清样本的特异性血清IgG抗体。针对 MERS冠状病毒和牛冠状病毒的病毒中和试验证实了实验结果。 Findings 50 of 50 (100%) sera from Omani camels and 15 of 105 (14%) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1/320 and 1/2560 for the Omani camel sera and between 1/20 and 1/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. 发现 全部50份阿曼骆驼血清样本(100%)和105份西班牙骆驼样本中的15份(14%)有针对MERS-CoV体冠状病毒的蛋白特异性抗体。来自欧洲绵羊,山羊,牛和其他骆驼等的血清样本则没有发现这种抗体。MERS冠状病毒中和抗体滴度在阿曼骆驼血清中的变化范围在1/320和1/2560之间,而在西班牙骆驼血清中的变化范围则在1/20和1/320之间。 没有发现牛冠状病毒抗体引起的交叉中和的相关证据。 Interpretation MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection. 说明 MERS冠状病毒或相关病毒已感染骆驼种群。来自阿曼不同地区的样本血清中的滴度和抗体阳性率表明了广泛的感染。 Funding European Union, European Centre For Disease Prevention and Control, Deutsche Forschungsgemeinschaft. 资助 欧盟,欧洲疾病预防控制中心,德意志研究联合会。

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