PLOS ONE2013-12-23 11:52 PM

A Built-In Strategy to Mitigate Transgene Spreading from Genetically Modified Corn 一个减缓基改玉米作物中转基因传播的内置策略

Abstract Transgene spreading is a major concern in cultivating genetically modified (GM) corn. Cross-pollination may cause the spread of transgenes from GM cornfields to conventional fields. Occasionally, seed lot contamination, volunteers, mixing during sowing, harvest, and trade can also lead to transgene escape. Obviously, new biological confinement technologies are highly desired to mitigate transgene spreading in addition to physical separation and isolation methods. In this study, we report the development of a built-in containment method to mitigate transgene spreading in corn. In this method, an RNAi cassette for suppressing the expression of the nicosulfuron detoxifying enzyme CYP81A9 and an expression cassette for the glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene G10 were constructed and transformed into corn via Agrobacterium-mediated transformation. The GM corn plants that were generated were found to be sensitive to nicosulfuron but resistant to glyphosate, which is exactly the opposite of conventional corn. Field tests demonstrated that GM corn plants with silenced CYP81A9 could be killed by applying nicosulfuron at 40 g/ha, which is the recommended dose for weed control in cornfields. This study suggests that this built-in containment method for controlling the spread of corn transgenes is effective and easy to implement. 论文摘要 转基因的传播控制是在培育转基因玉米中最受关注的问题之一。植物异花授粉可能是导致转基因从转基因玉米专属区域蔓延到传统玉米种植区域的原因。此外种子大面积的污染,“顺手客”们的随机携带传播,播种,收割,贸易过程中的不慎混入等偶然因素都能对转基因的蔓延散播起到推波助澜的作用。显而易见,除了采用分离隔绝等物理方法外,新的生物限制技术手段也成为控制拦截转基因传播的有力武器。在这项研究中,我们审视了针对玉米作物转基因传播的控制所研制的内置控制技术方法的发展历程。在该内置控制方法中,我们使用农杆菌介导法将能抑制烟嘧磺隆解毒酶CYP81A9基因表达和抗草甘膦酶基因G10基因表达的基因干扰盒分别构建和转植入玉米作物中。研究发现与传统玉米作物的植物特性恰好相反,随后生长出的转基因玉米作物对烟磺隆具有特定敏感性而对草甘膦则表现出了耐久抵抗力。实地种植试验证实了在施用浓度为40克/公顷的烟磺隆时,携带沉默CYP81A9基因的转基因玉米作物就能被杀死,这一最优有效浓度将成为控制玉米地野草肆长问题的推荐手段。此外研究人员根据试验结果认为内置控制技术具有高效和易于实施的有利特点,因而力荐将这一技术手段推广在玉米的转基因控制的实际运用中。

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