唧唧堂编辑二朵2014-01-09 5:02 AM

Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer 成功移植人体干细胞核生成人类胚胎干细胞

Abstract Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT)-ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we identified premature exit from meiosis in human oocytes and suboptimal activation as key factors that are responsible for these outcomes. Optimized SCNT approaches designed to circumvent these limitations allowed derivation of human NT-ESCs. When applied to premium quality human oocytes, NT-ESC lines were derived from as few as two oocytes. NT-ESCs displayed normal diploid karyotypes and inherited their nuclear genome exclusively from parental somatic cells. Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state. 论文摘要: 胚胎干细胞(ESCs),体细胞核移植(SCNT),细胞核移植胚胎干细胞((NT)-ESCs) 通过将人体细胞核移植入胚胎干细胞生成和病患基因匹配的胚胎干细胞已经被认为是将来人体疾病研究和治疗的方向。 过去生成 (NT)-ESCs 的失败很多可以归结为没有找到足够高质量的被移植的胚胎细胞。 我们发现这一结果的产生的原因是由于生成该胚胎细胞的卵子的减数分裂的过早结束而导致不太理想的活性状态。 所以如何优化细胞核移植方法来规避这些失败呢? 我们选取了最优质的卵子细胞来进行实验。 最终生成的NT-ESCs显示其拥有正常的二倍体核型,并且成功继承了来自母体细胞核的核基因因素。这一结果和胚胎型ESCs的显示结果类似,说明通过移植体细胞核进入人类卵子细胞从而生成人类胚胎细胞的实验有效。

KEYWORDS

SHARE & LIKE

COMMENTS

ABOUT THE AUTHOR

唧唧堂编辑二朵

jijitang editor flower 2

1 Following 7 Fans 0 Projects 149 Articles

SIMILAR ARTICLES

Abstract Dyscalculia, dyslexia, and specific language impairment (SLI) are relatively specific developmental learning disabilities in math, reading, a

Read More

Abstract Recent research in cognitive and developmental neuroscience is providing a new approach to the understanding of dyscalculia that emphasizes

Read More

Abstract Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an ap

Read More

Abstract Human collective behavior can vary from calm to panicked depending on social context. Using videos publicly available online, we study the hi

Read More

Abstract PurposeTo determine the contents of chicken nuggets from 2 national food chains. 目的 确定从2个全国食品连锁店获得的鸡块的内容。 BackgroundChicken nuggets have b

Read More

Abstract 论文摘要 Background Among adults, skipping meals is associated with excess body weight, hypertension, insulin resistance, and elevated fasting

Read More

AbstractTo the authors' knowledge, the role of restaurant menus as a vehicle for pathogens has not been explored. Menus, however, can pose as a vector

Read More

Maarten A. S. Boksem, PhDAbstractAlthough much progress has been made in relating brain activations to choice behaviour, evidence that neural measures

Read More