唧唧堂编辑二朵2014-02-06 5:15 AM

DNA barcoding detects contamination and substitution in North American herbal products DNA 条形码技术检测北美草药制剂中的污染和替换

Abstract Background Herbal products available to consumers in the marketplace may be contaminated or substituted with alternative plant species and fillers that are not listed on the labels. According to the World Health Organization, the adulteration of herbal products is a threat to consumer safety. Our research aimed to investigate herbal product integrity and authenticity with the goal of protecting consumers from health risks associated with product substitution and contamination. 背景 市场上提供给消费者的草药产品可能被替代性植物物种和填料污染或取代了,但这些替代品都未在标签上列出。据世界卫生组织表示草药产品的掺假行为造成了对消费者安全的威胁。我们的研究旨在探讨草药产品的完整性和真实性以保护消费者免受有关产品替代和污染造成的健康风险。 Methods We used DNA barcoding to conduct a blind test of the authenticity for (i) 44 herbal products representing 12 companies and 30 different species of herbs, and (ii) 50 leaf samples collected from 42 herbal species. Our laboratory also assembled the first standard reference material (SRM) herbal barcode library from 100 herbal species of known provenance that were used to identify the unknown herbal products and leaf samples. 方法 我们用DNA条码技术来实施对(Ⅰ)代表12家企业和30种不同草药的44种草药产品和(Ⅱ)50种从42类草药采集的叶子样品的真实性进行盲测。我们实验室也配备了首个采集自100类已知种源草药的标准参考物质(SRM)中草药条形码库,这些种源被用来识别未知草药产品和树叶样品。 Results We recovered DNA barcodes from most herbal products (91%) and all leaf samples (100%), with 95% species resolution using a tiered approach (rbcL + ITS2). Most (59%) of the products tested contained DNA barcodes from plant species not listed on the labels. Although we were able to authenticate almost half (48%) of the products, one-third of these also contained contaminants and or fillers not listed on the label. Product substitution occurred in 30/44 of the products tested and only 2/12 companies had products without any substitution, contamination or fillers. Some of the contaminants we found pose serious health risks to consumers. 结果 我们使用分层方法(rbcL+ ITS2),按95%的物种分辨率回收了大多数草药产品(91%)和所有的叶样品(100%)的DNA条形码。受测的大部分产品(59%)包含了在标签上未列出的植物种类的DNA条码。虽然我们能够鉴定几乎过半的产品(48%),但其中三分之一也包含了在标签上未列出的污染物和或填料。受测产品的替换发生率为30/44,且出产没有任何替换,污染或填料的受测产品的公司比率为2/12。某些已被发现的污染物会对消费者造成严重的健康风险。 Conclusions Most of the herbal products tested were of poor quality, including considerable product substitution, contamination and use of fillers. These activities dilute the effectiveness of otherwise useful remedies, lowering the perceived value of all related products because of a lack of consumer confidence in them. We suggest that the herbal industry should embrace DNA barcoding for authenticating herbal products through testing of raw materials used in manufacturing products. The use of an SRM DNA herbal barcode library for testing bulk materials could provide a method for 'best practices? in the manufacturing of herbal products. This would provide consumers with safe, high quality herbal products. 结论 大部分受测草药产品都质量很差,包括大量的产品替换,污染和填料使用。这些行径由于使得消费者失去了对其的信心,因而削弱了有效治疗措施的效用,降低了所有相关产品的感知价值。我们建议中草药行业应该运用DNA条形码技术来测试用作制造产品的原材料从而鉴别草药产品。运用SRM的DNA条形码草药库来测试块体材料可为草药产品制造的“最佳实践”提供一种新途径。这将为消费者提供安全,优质草本产品提供了保障。

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