Kevin Eggan2014-02-27 12:47 PM

Recipient cell nuclear factors are required for reprogramming by nuclear transfer通过细胞核移植的重编程需要受体细胞核因子 

Abstract

Nuclear transfer allows the reprogramming of somatic cells to totipotency. The cell cycle state of the donor and recipient cells, as well as their extent of differentiation, have each been cited as important determinants of reprogramming success. Here, we have used donor and recipient cells at various cell cycle and developmental stages to investigate the importance of these parameters. We found that many stages of the cell cycle were compatible with reprogramming as long as a sufficient supply of essential nuclear factors, such as Brg1, were retained in the recipient cell following enucleation. Consistent with this conclusion, the increased efficiency of reprogramming when using donor nuclei from embryonic cells could be explained, at least in part, by reintroduction of embryonic nuclear factors along with the donor nucleus. By contrast, cell cycle synchrony between the donor nucleus and the recipient cell was not required at the time of transfer, as long as synchrony was reached by the first mitosis. Our findings demonstrate the remarkable flexibility of the reprogramming process and support the importance of nuclear transcriptional regulators in mediating reprogramming.

论文摘要
核移植使得体细胞重编程为全能干细胞成为可能。供体和受体细胞的细胞周期状态以及他们的分化程度已分别被列为成功重编程重要的决定因素。我们在此使用处于不同细胞周期和发育阶段的供体和受体细胞来调查这些参数的重要性。我们发现细胞周期的多个阶段与重编程兼容,只要必要的核因素供应充足例如Brg1基因,在去核后仍能保留在受体细胞中。与该结论相一致,在当使用胚胎细胞的细胞核供体时,重编程效率的提高可以通过再引入胚胎细胞核因子和供体细胞核至少部分得到解释。与此相反,在保证截止至第一有丝分裂时能达到同步的前提下,供体核与受体细胞在核移植时互相的细胞周期不需要达到同步。我们的研究结果显示出重编程过程的显著灵活性并支持了在调解重编程时核转录调节的重要性。

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Kevin Eggan

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