Nature2014-06-26 1:30 PM

RNA–蛋白复合物中的组织方式 Protein-guided RNA dynamics during early ribosome assembly

论文摘要 

蛋白-RNA复合物的组装会涉及RNA结构的雕刻,因为蛋白是按顺序被结合在一起的,但对这些变化的详细情况的直接演示一直有困难。Sarah Woodson及同事采用先进的三色“荧光共振能量转移”(FRET)和模拟方法来研究在30S核糖体的组装中早期发生的事件。他们发现,当S4核糖体蛋白与16S rRNA发生相互作用时,存在一个稳定的、on-path、non-native中间体;而且该S4蛋白还能改变RNA螺旋动态,以帮助发生使随后的蛋白结合成为可能的构形变化。

Abstract 

The assembly of 30S ribosomes requires the precise addition of 20 proteins to the 16S ribosomal RNA. How early binding proteins change the ribosomal RNA structure so that later proteins may join the complex is poorly understood. Here we use single-molecule fluorescence resonance energy transfer (FRET) to observe real-time encounters between Escherichia coli ribosomal protein S4 and the 16S 5′ domain RNA at an early stage of 30S assembly. Dynamic initial S4–RNA complexes pass through a stable non-native intermediate before converting to the native complex, showing that non-native structures can offer a low free-energy path to protein–RNA recognition. Three-colour FRET and molecular dynamics simulations reveal how S4 changes the frequency and direction of RNA helix motions, guiding a conformational switch that enforces the hierarchy of protein addition. These protein-guided dynamics offer an alternative explanation for induced fit in RNA–protein complexes.

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