Stanford University2014-11-24 11:48 AM

A DNA-based molecular probe for optically reporting cellular traction forces

Abstract


We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution , can in principle be linked to virtually any surface , and obviate monitoring deformations of elastic substrates . 


TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces . 


We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges .



Full Article:

http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.3145.html

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